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Addgene

S48I48 ELP
(Plasmid #68394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET25b+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5547
  • Total vector size (bp) 6925
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S48I48 ELP
  • Alt name
    elastin-like polypeptide
  • Alt name
    G(VPGSG)48(VPGIG)48Y
  • Species
    Synthetic
  • Insert Size (bp)
    1500
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    S48I48 ELP was a gift from Andrew MacKay (Addgene plasmid # 68394 ; http://n2t.net/addgene:68394 ; RRID:Addgene_68394)

  • For your References section:

    Biodegradation of elastin-like polypeptide nanoparticles. Shah M, Hsueh PY, Sun G, Chang HY, Janib SM, MacKay JA. Protein Sci. 2012 Jun;21(6):743-50. doi: 10.1002/pro.2063. Epub 2012 May 14. 10.1002/pro.2063 PubMed 22434766
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