knob-s48i48
(Plasmid
#68391)
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PurposeKnob protein fused to elastin-like polypeptide (S48I48)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePet25b+
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5547
- Total vector size (bp) 7531
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKnob-S48I48
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Alt nameknob-ELP
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SpeciesSynthetic
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Insert Size (bp)2100
- Promoter T7
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Tag
/ Fusion Protein
- Knob (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
knob-s48i48 was a gift from Andrew MacKay (Addgene plasmid # 68391 ; http://n2t.net/addgene:68391 ; RRID:Addgene_68391) -
For your References section:
Design and cellular internalization of genetically engineered polypeptide nanoparticles displaying adenovirus knob domain. Sun G, Hsueh PY, Janib SM, Hamm-Alvarez S, MacKay JA. J Control Release. 2011 Oct 30;155(2):218-26. doi: 10.1016/j.jconrel.2011.06.010. Epub 2011 Jun 14. 10.1016/j.jconrel.2011.06.010 PubMed 21699930
Map uploaded by the depositor.
