pJH110
(Plasmid
#68376)
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Purpose(Empty Backbone) Clone cassette to replace RFP for integration into C31 landing pad strain
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68376 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepK19mobsacB
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains an R98K mutation in the Gm resistance cassette. The depositor states that this mutation should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH110 was a gift from Trevor Charles (Addgene plasmid # 68376 ; http://n2t.net/addgene:68376 ; RRID:Addgene_68376) -
For your References section:
Site-specific bacterial chromosome engineering: PhiC31 integrase mediated cassette exchange (IMCE). Heil JR, Cheng J, Charles TC. J Vis Exp. 2012 Mar 16;(61). pii: 3698. doi: 10.3791/3698. 10.3791/3698 PubMed 22453661
