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Addgene

CAS9PBKS
(Plasmid #68371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBKS
  • Backbone manufacturer
    Stratagene/Agilent
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    S.pyogenes
  • Promoter CbAkt
  • Tag / Fusion Protein
    • 2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13-Rev
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cas9-2A-EGFP was subcloned from pSpCas9(BB)-2A-GFP (PX458) from Feng Zhang (Addgene plasmid # 48138)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAS9PBKS was a gift from Eric-Paul Bennett (Addgene plasmid # 68371 ; http://n2t.net/addgene:68371 ; RRID:Addgene_68371)

  • For your References section:

    Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis. Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M. Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16. 10.1038/nprot.2016.165 PubMed 28207001
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