U6GRNA
(Plasmid
#68370)
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Purpose(Empty Backbone) gRNA scaffold with hU6 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSpCas9(BB)-2A-GFP (PX458)
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Backbone manufacturerFeng Zhang (Addgene plasmid # 48138)
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Modifications to backbonedigested px458 with KpnI/NotI and religated plasmid
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Vector typeMammalian Expression
- Promoter hU6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer hU6-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
U6GRNA was a gift from Eric-Paul Bennett (Addgene plasmid # 68370 ; http://n2t.net/addgene:68370 ; RRID:Addgene_68370) -
For your References section:
Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis. Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M. Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16. 10.1038/nprot.2016.165 PubMed 28207001
