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Addgene

EPB104
(Plasmid #68369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GENSCRIPT CORPORATION
  • Backbone size w/o insert (bp) 2399
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    249
  • GenBank ID
    gb|M14486.1|HUMUG6
  • Entrez Gene
    RNU6-1 (a.k.a. RNU6, RNU6A, U6, U6-1)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EPB104 was a gift from Eric-Paul Bennett (Addgene plasmid # 68369 ; http://n2t.net/addgene:68369 ; RRID:Addgene_68369)

  • For your References section:

    Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis. Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M. Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16. 10.1038/nprot.2016.165 PubMed 28207001
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