Skip to main content
Addgene

BAP1-010
(Plasmid #68366)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI-puro
  • Backbone manufacturer
    Dr. J. Mirkovitch, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland
  • Backbone size w/o insert (bp) 5224
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BAP1
  • Alt name
    UCHL2
  • Alt name
    hucep-6
  • Alt name
    HUCEP-13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2190
  • Mutation
    delta 221-238, splice variant found in human mesothelioma cell line
  • GenBank ID
    8314 NM_004656.3
  • Entrez Gene
    BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer chim-int-F
  • 3′ sequencing primer EBV reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Described in Vega database as BAP1-010 (OTTHUMT00000351150), encodes a 711 aa long protein

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BAP1-010 was a gift from Emanuela Felley-Bosco & Rolf Stahel (Addgene plasmid # 68366 ; http://n2t.net/addgene:68366 ; RRID:Addgene_68366)

  • For your References section:

    A novel BRCA1-associated protein-1 isoform affects response of mesothelioma cells to drugs impairing BRCA1-mediated DNA repair. Parrotta R, Okonska A, Ronner M, Weder W, Stahel R, Penengo L, Felley-Bosco E. J Thorac Oncol. 2017 Apr 4. pii: S1556-0864(17)30274-5. doi: 10.1016/j.jtho.2017.03.023. 10.1016/j.jtho.2017.03.023 PubMed 28389374
Related items:
Commonly requested with: