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BAP1-001
(Plasmid #68365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-puro
  • Backbone manufacturer
    Dr. J. Mirkovitch, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland
  • Backbone size w/o insert (bp) 5224
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BAP1
  • Alt name
    UCHL2
  • Alt name
    hucep-6
  • Alt name
    HUCEP-13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2190
  • GenBank ID
    8314 NM_004656.3
  • Entrez Gene
    BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer chim-int-F
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes a 729 aa long protein

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BAP1-001 was a gift from Emanuela Felley-Bosco & Rolf Stahel (Addgene plasmid # 68365 ; http://n2t.net/addgene:68365 ; RRID:Addgene_68365)

  • For your References section:

    A novel BRCA1-associated protein-1 isoform affects response of mesothelioma cells to drugs impairing BRCA1-mediated DNA repair. Parrotta R, Okonska A, Ronner M, Weder W, Stahel R, Penengo L, Felley-Bosco E. J Thorac Oncol. 2017 Apr 4. pii: S1556-0864(17)30274-5. doi: 10.1016/j.jtho.2017.03.023. 10.1016/j.jtho.2017.03.023 PubMed 28389374
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