pDestattB/CY (pCM327)
(Plasmid
#68341)
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Purpose(Empty Backbone) Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestattB
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Backbone manufacturerMosimann lab, Addgene plasmid #68313
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Modifications to backbonecloned by introducing the Asp718-flanked α-crystallin:Venus (YFP) cassette (Hesselson et al., 2009) via blunt cloning into the blunted XhoI site in pDestattB (pCM268).
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Vector typeZebrafish transgenesis
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDestattB/CY (pCM327) was a gift from Christian Mosimann & Leonard Zon (Addgene plasmid # 68341 ; http://n2t.net/addgene:68341 ; RRID:Addgene_68341) -
For your References section:
Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system. Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI. Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 Jul 3. 10.1002/dvdy.23989 PubMed 23723152