PX394 Neisseria cinerea Cas9
(Plasmid #68331)

• Purpose: Neisseria cinerea Cas9
• Depositing Lab: Feng Zhang
• Publication: Feng Zhang lab bacterial Cas9 orthologs (unpublished)

Backbone

• Vector backbone: pDREAM
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NcCas9
• Alt name: Neisseria cinerea Cas9
• Species: Synthetic; Neisseria cinerea
• Promoter: CMV
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')

Resource Information

• Supplemental Documents:
  • PX394-18 Neisseria cinerea Cas9
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry