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Addgene

pDestattB (pCM268)
(Plasmid #68313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWB.attB
  • Modifications to backbone
    the white+ gene was removed by digest with EcoRV and NheI and subsequent blunt re-ligation, which created pattB (pCM267). The Multisite Gateway cassette from Tol2kit vector #394 was then introduced into pattB via XhoI/Asp718 double digest.
  • Vector type
    Zebrafish transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestattB (pCM268) was a gift from Christian Mosimann & Leonard Zon (Addgene plasmid # 68313 ; http://n2t.net/addgene:68313 ; RRID:Addgene_68313)

  • For your References section:

    Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system. Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI. Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 Jul 3. 10.1002/dvdy.23989 PubMed 23723152
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