pcDNA3.1 phiC31
(Plasmid #68310)

• Purpose: In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).
• Depositing Lab: Konrad Basler
• Publication: Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7. Epub 2007 Feb 22.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Vector type: RNA transcription
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phiC31
• Alt name: integrase
• Alt name: serine family SSR phiC31 integrase
• Insert Size (bp): 1100
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pcDNA3.1 phiC31 genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry