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pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
(Plasmid #68280)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mkk2DS NLS mCherry
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    MKK2 (a.k.a. YPL140C, LPI6, SSP33)
  • Promoter RPS2
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry was a gift from Serge Pelet (Addgene plasmid # 68280 ; http://n2t.net/addgene:68280 ; RRID:Addgene_68280)

  • For your References section:

    Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors. Durandau E, Aymoz D, Pelet S. BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z. 10.1186/s12915-015-0163-z [pii] PubMed 26231587
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