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Addgene

pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
(Plasmid #68278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ste7DS NLS mCitrine
  • Species
    Synthetic
  • Promoter pRPS2
  • Tag / Fusion Protein
    • mCitrine (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pED73 pRS304 pRPS2-SteDS-NLS-mCitrine was a gift from Serge Pelet (Addgene plasmid # 68278 ; http://n2t.net/addgene:68278 ; RRID:Addgene_68278)

  • For your References section:

    Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors. Durandau E, Aymoz D, Pelet S. BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z. 10.1186/s12915-015-0163-z [pii] PubMed 26231587
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