pDGB1alpha2_SF (GB0107)
(Plasmid #68233)

• Purpose: Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector
• Depositing Lab: Diego Orzaez
• Publication: Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Backbone

• Vector backbone: pDGB1_alpha2
• Backbone manufacturer: self-made; derived from pGreenII generated at the JIC
• Vector type: Synthetic Biology ; Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Solanum lycopersicum intergenic region
• Insert Size (bp): 150
• Mutation: BsaI and BsmBI sites removed

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: caacctctcgggcttctgga

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

insert can be released with BsmBI