pRosea (GB0026)
(Plasmid #68194)

• Purpose: Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part
• Depositing Lab: Diego Orzaez
• Publication: Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Backbone

• Vector backbone: pUPD
• Backbone manufacturer: self-made; derived from pGEMT-Easy manufactured by Promega
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: A. majus Rosea coding region
• Insert Size (bp): 660
• Mutation: BsaI and BsmBI sites removed

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

insert can be released with BsaI