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Addgene

0.22 hA33 luciferase
(Plasmid #68157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A33
  • Alt name
    GPA33
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    187
  • Mutation
    starts at -222 relative to ATG
  • GenBank ID
    10223
  • Entrez Gene
    GPA33 (a.k.a. A33)
  • Promoter human GPA33 promoter
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer lucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    0.22 hA33 luciferase was a gift from Joan Heath (Addgene plasmid # 68157 ; http://n2t.net/addgene:68157 ; RRID:Addgene_68157)

  • For your References section:

    Analysis of the regulation of the A33 antigen gene reveals intestine-specific mechanisms of gene expression. Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, Viars CS, Czekay S, Arden KC, Heath JK. J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11. 10.1074/jbc.M204865200 PubMed 12114523
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