pCer-C3-Plk1-K82R
(Plasmid #68134)

• Purpose: Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells
• Depositing Lab: Catherine Lindon
• Publication: pCer-C3-Plk1 (unpublished)

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6500
• Modifications to backbone: EGFP swapped for Cerulean
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Polo-like kinase 1
• Alt name: Plk1
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: K82R kinase-dead version
• GenBank ID: NM_005030.4
• Entrez Gene: PLK1 (a.k.a. PLK, STPK13)
• Promoter: CMV
• Tag / Fusion Protein:
  • Cerulean (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xba1 (destroyed during cloning)
• 5′ sequencing primer: GFP-FP
• 3′ sequencing primer: pEGFP-C2-RP

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Erich Nigg
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry