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Addgene

pcdna nesprin HL
(Plasmid #68128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcdna 3.1 -
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8010
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nesprin-2
  • Alt name
    SYNE2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2622
  • Mutation
    deleted actin-binding domain
  • Entrez Gene
    Syne2 (a.k.a. 6820443O06Rik, Cpfl8, D12Ertd777e, KASH2, NUA, Nesp2g, dice, mKIAA1011)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna nesprin HL was a gift from Daniel Conway (Addgene plasmid # 68128 ; http://n2t.net/addgene:68128 ; RRID:Addgene_68128)

  • For your References section:

    Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to Myosin-Dependent Tension. Arsenovic PT, Ramachandran I, Bathula K, Zhu R, Narang JD, Noll NA, Lemmon CA, Gundersen GG, Conway DE. Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014. 10.1016/j.bpj.2015.11.014 PubMed 26745407
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