pcdna nesprin TS
(Plasmid #68127)

• Purpose: nesprin tension sensor
• Depositing Lab: Daniel Conway
• Publication: Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014.

Backbone

• Vector backbone: pcdna 3.1 +
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 9476
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse nesprin mini 2G
• Alt name: SYNE2
• Species: M. musculus (mouse)
• Insert Size (bp): 4095
• Entrez Gene: Syne2 (a.k.a. 6820443O06Rik, Cpfl8, D12Ertd777e, KASH2, NUA, Nesp2g, dice, mKIAA1011)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry