pRT802
(Plasmid #68113)

• Purpose: Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.
• Depositing Lab: Maggie Smith
• Publication: Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

Backbone

• Vector backbone: pSET152
• Modifications to backbone: PhiC31 int/attP replaced with phiBT1 int/attP
• Vector type: Streptomyces integrating vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: aac3(IV)
• Alt name: apramycin fragment
• Species: E. coli
• Insert Size (bp): 29

Gene/Insert 2

• Gene/Insert name: aph11
• Alt name: aminoglycoside phosphotransferase
• Species: transposon Tn5
• Insert Size (bp): 925

Gene/Insert 3

• Gene/Insert name: oriT
• Alt name: origin of transfer
• Species: E. coli

Gene/Insert 4

• Gene/Insert name: attP
• Alt name: phage attachment site
• Species: bacteriophage phiBT1
• Insert Size (bp): 51

Gene/Insert 5

• Gene/Insert name: int
• Alt name: phiBT1 integrase
• Species: bacteriophage phiBT1
• Insert Size (bp): 1430

Resource Information

• Supplemental Documents:
  • pRT802.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For research using this plasmid, please acknowledge the depositing laboratory and associated citation.