pMS82
(Plasmid #68110)

• Purpose: Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication
• Depositing Lab: Maggie Smith
• Publication: Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

Backbone

• Vector backbone: pSET152
• Modifications to backbone: PhiC31 int/attP replaced with phiBT1 int/attP. aac3(IV) gene mostly deleted and hyg gene inserted.
• Vector type: Streptomyces integrating vector

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: aac3(IV)
• Alt name: apramycin resistance (fragment)
• Species: E. coli
• Insert Size (bp): 26
• Mutation: Fragment

Gene/Insert 2

• Gene/Insert name: Hyg
• Alt name: Hygromycin resistance
• Insert Size (bp): 998

Gene/Insert 3

• Gene/Insert name: oriT
• Alt name: origin of transfer
• Species: E. coli
• Insert Size (bp): 111

Gene/Insert 4

• Gene/Insert name: attP
• Alt name: phage attachment site
• Species: bacteriophage phiBT1
• Insert Size (bp): 51

Gene/Insert 5

• Gene/Insert name: int
• Alt name: phiBT1 Integrase
• Species: bacteriophage phiBT1
• Insert Size (bp): 1785

Resource Information

• Supplemental Documents:
  • pMS82.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For research using this plasmid, please acknowledge the depositing laboratory and associated citation.