oxVenus
(Plasmid
#68067)
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Purpose(Empty Backbone) C-terminal fusion to oxVenus
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Modifications to backboneoxFP inserted via AgeI/NotI restriction sites, removing EGFP
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- oxVenus (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from Venus. Human codon optimized and contains the sfGFP and cysteine to serine mutations.
Excitation/Emission 514/526
Brightness 44 (product of extinction coefficient and quantum yield and divided by 1,000).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
oxVenus was a gift from Erik Snapp (Addgene plasmid # 68067 ; http://n2t.net/addgene:68067 ; RRID:Addgene_68067) -
For your References section:
A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. 10.1038/ncomms8670 PubMed 26158227