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Addgene

pBLV B4
(Plasmid #68057)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIDTSmart-Kan
  • Backbone manufacturer
    Integrated DNA Technologies
  • Backbone size w/o insert (bp) 1962
  • Total vector size (bp) 2013
  • Modifications to backbone
    Xho1 and Xba1 sites were inserted into the pIDTSmart-Kan backbone.
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BLV-miR-B4
  • gRNA/shRNA sequence
    N/A
  • Species
    Bovine leukemia virus
  • GenBank ID
    NC_001414

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer M13 Foward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBLV B4 was a gift from Christopher Sullivan (Addgene plasmid # 68057 ; http://n2t.net/addgene:68057 ; RRID:Addgene_68057)

  • For your References section:

    Identification of tri-phosphatase activity in the biogenesis of retroviral microRNAs and RNAP III-generated shRNAs. Burke JM, Bass CR, Kincaid RP, Sullivan CS. Nucleic Acids Res. 2014 Dec 16;42(22):13949-62. doi: 10.1093/nar/gku1247. Epub 2014 Nov 26. 10.1093/nar/gku1247 PubMed 25428356
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