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Addgene

pcDNA3.1dsRluc
(Plasmid #68053)

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Item Catalog # Description Quantity Price (USD)
Plasmid 68053 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone size w/o insert (bp) 5428
  • Modifications to backbone
    neomycin resistance replaced with puromycin resistance.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1dsRluc was a gift from Christopher Sullivan (Addgene plasmid # 68053 ; http://n2t.net/addgene:68053 ; RRID:Addgene_68053)

  • For your References section:

    Evolutionarily conserved function of a viral microRNA. Seo GJ, Fink LH, O'Hara B, Atwood WJ, Sullivan CS. J Virol. 2008 Oct;82(20):9823-8. doi: 10.1128/JVI.01144-08. Epub 2008 Aug 6. 10.1128/JVI.01144-08 PubMed 18684810
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