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Addgene

pISK HSUR4
(Plasmid #68052)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIDTSmart-Kan
  • Backbone manufacturer
    Integrated DNA Technologies
  • Backbone size w/o insert (bp) 1962
  • Total vector size (bp) 2484
  • Modifications to backbone
    Xho1 and Xba1 sites were inserted into the pIDTSmart-Kan backbone.
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HSUR4
  • gRNA/shRNA sequence
    N/A
  • Species
    Herpesvirus saimiri 2
  • GenBank ID
    X64346.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer M13 Foward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISK HSUR4 was a gift from Christopher Sullivan (Addgene plasmid # 68052 ; http://n2t.net/addgene:68052 ; RRID:Addgene_68052)

  • For your References section:

    Noncanonical microRNA (miRNA) biogenesis gives rise to retroviral mimics of lymphoproliferative and immunosuppressive host miRNAs. Kincaid RP, Chen Y, Cox JE, Rethwilm A, Sullivan CS. mBio. 2014 Apr 8;5(2):e00074. doi: 10.1128/mBio.00074-14. 10.1128/mBio.00074-14 PubMed 24713319
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