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pET41-GST-KaiC
(Plasmid #68051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68051 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET41a(+)
  • Total vector size (bp) 7500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KaiC
  • Species
    Synechococcus elongatus
  • Insert Size (bp)
    1500
  • GenBank ID
    CP000100
  • Promoter T7
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 6xHis (N terminal on backbone)
    • S-Tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PreScission (GE Healthcare) Protease cutting site is introduced before KaiC. KaiC is cloned between NcoI and HiNdII. Use BL21DE3 and GST column for protein purification. Use PreScission Protease to cut GST tag off.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET41-GST-KaiC was a gift from Susan Golden (Addgene plasmid # 68051 ; http://n2t.net/addgene:68051 ; RRID:Addgene_68051)

  • For your References section:

    Oxidized quinones signal onset of darkness directly to the cyanobacterial circadian oscillator. Kim YI, Vinyard DJ, Ananyev GM, Dismukes GC, Golden SS. Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17765-9. doi: 10.1073/pnas.1216401109. Epub 2012 Oct 15. 10.1073/pnas.1216401109 PubMed 23071342
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