pMyo-2::oxCerulean-oxVenus::Lgg-1
(Plasmid
#68043)
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PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagy
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCFJ150
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Backbone manufacturerErik Jorgensen
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepMyo-2::oxCerulean-oxVenus::Lgg-1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMyo-2::oxCerulean-oxVenus::Lgg-1 was a gift from Dana Miller (Addgene plasmid # 68043 ; http://n2t.net/addgene:68043 ; RRID:Addgene_68043) -
For your References section:
Tissue-specific autophagy responses to aging and stress in C. elegans. Chapin HA, Okada M, Merz AJ, Miller DL. Aging (Albany NY). 2015 Jun 30. 100765 [pii] PubMed 26142908