pmPB3-VSV
(Plasmid
#68039)
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PurposeExpresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 11200
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Modifications to backboneHGF signal sequence
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlexin-B3
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SpeciesM. musculus (mouse)
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Insert Size (bp)5900
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Entrez GenePlxnb3 (a.k.a. AI451018, Plxn6)
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Tag
/ Fusion Protein
- VSV (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmPB3-VSV was a gift from Roland Friedel (Addgene plasmid # 68039 ; http://n2t.net/addgene:68039 ; RRID:Addgene_68039) -
For your References section:
Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development. Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani H, Tessier-Lavigne M, Wurst W, Friedel RH. Mol Cell Neurosci. 2011 Feb;46(2):419-31. doi: 10.1016/j.mcn.2010.11.005. Epub 2010 Nov 29. 10.1016/j.mcn.2010.11.005 PubMed 21122816