pCRII-Sema4b-ISH-2kb
(Plasmid
#68030)
-
PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 68030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCRII-TOPO
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3973
-
Vector typemRNA In situ hybridization probe
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSemaphorin-4B
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)2022
-
Entrez GeneSema4b (a.k.a. SemC, Semac, mKIAA1745)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCRII-Sema4b-ISH-2kb was a gift from Roland Friedel (Addgene plasmid # 68030 ; http://n2t.net/addgene:68030 ; RRID:Addgene_68030) -
For your References section:
Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development. Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani H, Tessier-Lavigne M, Wurst W, Friedel RH. Mol Cell Neurosci. 2011 Feb;46(2):419-31. doi: 10.1016/j.mcn.2010.11.005. Epub 2010 Nov 29. 10.1016/j.mcn.2010.11.005 PubMed 21122816