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pCRII-Sema4a-ISH-2kb
(Plasmid #68029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3973
  • Vector type
    mRNA In situ hybridization probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Semaphorin-4A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2087
  • Mutation
    a 2kb cDNA fragment for ISH probe synthesis
  • Entrez Gene
    Sema4a (a.k.a. AI132332, SemB, Semab)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-Sema4a-ISH-2kb was a gift from Roland Friedel (Addgene plasmid # 68029 ; http://n2t.net/addgene:68029 ; RRID:Addgene_68029)

  • For your References section:

    Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development. Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani H, Tessier-Lavigne M, Wurst W, Friedel RH. Mol Cell Neurosci. 2011 Feb;46(2):419-31. doi: 10.1016/j.mcn.2010.11.005. Epub 2010 Nov 29. 10.1016/j.mcn.2010.11.005 PubMed 21122816
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