pRetroX SgK269 WT
(Plasmid #68028)

• Purpose: expression of Sgk269 in mammalian cells
• Depositing Lab: Roger Daly
• Publication: Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. doi: 10.1158/0008-5472.CAN-12-1472. Epub 2013 Feb 1.

Backbone

• Vector backbone: pRetroX-IRES-DsRed
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 11320
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PEAK1
• Alt name: Sgk269
• Species: H. sapiens (human)
• Insert Size (bp): 5449
• Entrez Gene: PEAK1 (a.k.a. SGK269)
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • FLAG/myc (C terminal on insert)
  • IRES-DsRed (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EF-1a-F
• 3′ sequencing primer: pCDH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. David Croucher at the Garvan Institute, Sydney

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry