pEGFP-Anillin
(Plasmid #68027)

• Purpose: Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1
• Depositing Lab: Michael Glotzer
• Publication: Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2007 Dec 27.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7976
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Anillin
• Alt name: hsAnillin
• Species: H. sapiens (human)
• Insert Size (bp): 3264
• Mutation: silent mutations at 798 5'-TGCC TCTTTGAATAAA-3' 814 to 5' -CGCAAGCTTAAACAAG-3' for RNAi resistance. D278A
• GenBank ID: BC070066.1
• Entrez Gene: ANLN (a.k.a. FSGS8, Scraps, scra)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CATGGACGAGCTGTACAAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry