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Addgene

pABD1275
(Plasmid #68017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23b
  • Backbone manufacturer
    Novagen; EMD Millipore
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 5109
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PHY-INTERACTING FACTOR 1 (PIF1)
  • Alt name
    PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 (PIL5)
  • Species
    A. thaliana (mustard weed); AT2G20180
  • Insert Size (bp)
    1443
  • Mutation
    Codon Optimized for bacterial expression (GenScript)
  • Entrez Gene
    PIL5 (a.k.a. AT2G20180, PHY-INTERACTING FACTOR 1, PIF1, T2G17.2, T2G17_2, phytochrome interacting factor 3-like 5)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pABD1275 was a gift from Bruce Downie (Addgene plasmid # 68017 ; http://n2t.net/addgene:68017 ; RRID:Addgene_68017)

  • For your References section:

    KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1. Majee M, Kumar S, Kathare PK, Wu S, Gingerich D, Nayak NR, Salaita L, Dinkins R, Martin K, Goodin M, Dirk LMA, Lloyd TD, Zhu L, Chappell J, Hunt AG, Vierstra R, Huq E, Downie AB. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9. 10.1073/pnas.1711919115 PubMed 29632208
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