pRosa26-EF1a-hCas9IRESneo Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 67987 with PacI and NheI-HF.
We predict the following banding patterns:
PacI single digest: 19kb
NheI-HF single digest: 19kb
PacI / NheI-HF double digest: 13kb & 6kb
We see the expected result.
