pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W Citations (32)
Originally described in: A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. PubMed Journal
Articles Citing pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
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| Genome-scale identification of cellular pathways required for cell surface recognition. Sharma S, Bartholdson SJ, Couch ACM, Yusa K, Wright GJ. Genome Res. 2018 Sep;28(9):1372-1382. doi: 10.1101/gr.231183.117. Epub 2018 Jun 18. PubMed |
| Endogenous HIF2A reporter systems for high-throughput functional screening. Zaini MN, Patel SA, Syafruddin SE, Rodrigues P, Vanharanta S. Sci Rep. 2018 Aug 13;8(1):12063. doi: 10.1038/s41598-018-30499-2. PubMed |
| Predicting the mutations generated by repair of Cas9-induced double-strand breaks. Allen F, Crepaldi L, Alsinet C, Strong AJ, Kleshchevnikov V, De Angeli P, Palenikova P, Khodak A, Kiselev V, Kosicki M, Bassett AR, Harding H, Galanty Y, Munoz-Martinez F, Metzakopian E, Jackson SP, Parts L. Nat Biotechnol. 2018 Nov 27. pii: nbt.4317. doi: 10.1038/nbt.4317. PubMed |
| Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays. Deshpande A, Chen BR, Zhao L, Saddoris K, Kerr M, Zhu N, Mali P, Deshpande AJ. J Vis Exp. 2019 Jan 7;(143). doi: 10.3791/58710. PubMed |
| Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens. Behan FM, Iorio F, Picco G, Goncalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ. Nature. 2019 Apr;568(7753):511-516. doi: 10.1038/s41586-019-1103-9. Epub 2019 Apr 10. PubMed |
| A molecular triage process mediated by RING finger protein 126 and BCL2-associated athanogene 6 regulates degradation of G0/G1 switch gene 2. Kamikubo K, Kato H, Kioka H, Yamazaki S, Tsukamoto O, Nishida Y, Asano Y, Imamura H, Kawahara H, Shintani Y, Takashima S. J Biol Chem. 2019 Oct 4;294(40):14562-14573. doi: 10.1074/jbc.RA119.008544. Epub 2019 Aug 1. PubMed |
| Replication stress induces mitotic death through parallel pathways regulated by WAPL and telomere deprotection. Masamsetti VP, Low RRJ, Mak KS, O'Connor A, Riffkin CD, Lamm N, Crabbe L, Karlseder J, Huang DCS, Hayashi MT, Cesare AJ. Nat Commun. 2019 Sep 17;10(1):4224. doi: 10.1038/s41467-019-12255-w. PubMed |
| Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens. Sharma S, Wright GJ. J Vis Exp. 2020 Jun 6;(160). doi: 10.3791/60803. PubMed |
| Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system. Gratacap RL, Regan T, Dehler CE, Martin SAM, Boudinot P, Collet B, Houston RD. BMC Biotechnol. 2020 Jun 23;20(1):35. doi: 10.1186/s12896-020-00626-x. PubMed |
| Combinatorial CRISPR screen identifies fitness effects of gene paralogues. Thompson NA, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, Behan F, Goncalves E, Speak A, Iorio F, Hewinson J, Harle V, Robertson H, Anderson E, Fu B, Yang F, Zagnoli-Vieira G, Chapman P, Del Castillo Velasco-Herrera M, Garnett MJ, Jackson SP, Adams DJ. Nat Commun. 2021 Feb 26;12(1):1302. doi: 10.1038/s41467-021-21478-9. PubMed |
RNA Binding Proteins As Regulators of Oxidative Stress Identified by a Targeted CRISPR-Cas9 Single Guide RNA Library.
Turner DJ, Turner M.
CRISPR J. 2021 Jun;4(3):427-437. doi: 10.1089/crispr.2020.0116. Epub 2021 Jun 4.
PubMed
Associated Plasmids |
| Nuclear NAD(+) homeostasis governed by NMNAT1 prevents apoptosis of acute myeloid leukemia stem cells. Shi X, Jiang Y, Kitano A, Hu T, Murdaugh RL, Li Y, Hoegenauer KA, Chen R, Takahashi K, Nakada D. Sci Adv. 2021 Jul 21;7(30). pii: 7/30/eabf3895. doi: 10.1126/sciadv.abf3895. Print 2021 Jul. PubMed |
| Mutational synergy during leukemia induction remodels chromatin accessibility, histone modifications and three-dimensional DNA topology to alter gene expression. Yun H, Narayan N, Vohra S, Giotopoulos G, Mupo A, Madrigal P, Sasca D, Lara-Astiaso D, Horton SJ, Agrawal-Singh S, Meduri E, Basheer F, Marando L, Gozdecka M, Dovey OM, Castillo-Venzor A, Wang X, Gallipoli P, Muller-Tidow C, Osborne CS, Vassiliou GS, Huntly BJP. Nat Genet. 2021 Oct;53(10):1443-1455. doi: 10.1038/s41588-021-00925-9. Epub 2021 Sep 23. PubMed |
| Efficient Inhibition of HIV Using CRISPR/Cas13d Nuclease System. Nguyen H, Wilson H, Jayakumar S, Kulkarni V, Kulkarni S. Viruses. 2021 Sep 16;13(9). pii: v13091850. doi: 10.3390/v13091850. PubMed |
A functional genetic toolbox for human tissue-derived organoids.
Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL.
Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886.
PubMed
Associated Plasmids |
| In vivo genome-wide CRISPR screens identify SOCS1 as intrinsic checkpoint of CD4(+) TH1 cell response. Sutra Del Galy A, Menegatti S, Fuentealba J, Lucibello F, Perrin L, Helft J, Darbois A, Saitakis M, Tosello J, Rookhuizen D, Deloger M, Gestraud P, Socie G, Amigorena S, Lantz O, Menger L. Sci Immunol. 2021 Dec 3;6(66):eabe8219. doi: 10.1126/sciimmunol.abe8219. Epub 2021 Dec 3. PubMed |
| Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions. Meek S, Watson T, Eory L, McFarlane G, Wynne FJ, McCleary S, Dunn LEM, Charlton EM, Craig C, Shih B, Regan T, Taylor R, Sutherland L, Gossner A, Chintoan-Uta C, Fletcher S, Beard PM, Hassan MA, Grey F, Hope JC, Stevens MP, Nowak-Imialek M, Niemann H, Ross PJ, Tait-Burkard C, Brown SM, Lefevre L, Thomson G, McColl BW, Lawrence AB, Archibald AL, Steinbach F, Crooke HR, Gao X, Liu P, Burdon T. BMC Biol. 2022 Jan 14;20(1):14. doi: 10.1186/s12915-021-01217-8. PubMed |
| Mammalian HEMK1 methylates glutamine residue of the GGQ motif of mitochondrial release factors. Fang Q, Kimura Y, Shimazu T, Suzuki T, Yamada A, Dohmae N, Iwasaki S, Shinkai Y. Sci Rep. 2022 Mar 8;12(1):4104. doi: 10.1038/s41598-022-08061-y. PubMed |
| Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer. Gogleva A, Polychronopoulos D, Pfeifer M, Poroshin V, Ughetto M, Martin MJ, Thorpe H, Bornot A, Smith PD, Sidders B, Dry JR, Ahdesmaki M, McDermott U, Papa E, Bulusu KC. Nat Commun. 2022 Mar 29;13(1):1667. doi: 10.1038/s41467-022-29292-7. PubMed |
| Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells. Martins C, Silva M, Rasbach E, Singh P, Itoh Y, Williams JB, Statham E, Meurer A, Martinez DV, Brandenburg A, Heppt MV, Barthel SR, Schatton T. Sci Rep. 2022 Jul 21;12(1):12491. doi: 10.1038/s41598-022-16776-1. PubMed |
| HIRA loss transforms FH-deficient cells. Valcarcel-Jimenez L, Rogerson C, Yong C, Schmidt C, Yang M, Cremades-Rodelgo M, Harle V, Offord V, Wong K, Mora A, Speed A, Caraffini V, Tran MGB, Maher ER, Stewart GD, Vanharanta S, Adams DJ, Frezza C. Sci Adv. 2022 Oct 21;8(42):eabq8297. doi: 10.1126/sciadv.abq8297. Epub 2022 Oct 21. PubMed |
| Single-cell CRISPR immune screens reveal immunological roles of tumor intrinsic factors. Hou J, Liang S, Xu C, Wei Y, Wang Y, Tan Y, Sahni N, McGrail DJ, Bernatchez C, Davies M, Li Y, Chen R, Yi SS, Chen Y, Yee C, Chen K, Peng W. NAR Cancer. 2022 Dec 9;4(4):zcac038. doi: 10.1093/narcan/zcac038. eCollection 2022 Dec. PubMed |
| Expanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability. Chen ACH, Lee YL, Ruan H, Huang W, Fong SW, Tian S, Lee KC, Wu GM, Tan Y, Wong TCH, Wu J, Zhang W, Cao D, Chow JFC, Liu P, Yeung WSB. Adv Sci (Weinh). 2023 Apr;10(11):e2204797. doi: 10.1002/advs.202204797. Epub 2023 Feb 12. PubMed |
| Optimized whole-genome CRISPR interference screens identify ARID1A-dependent growth regulators in human induced pluripotent stem cells. Usluer S, Hallast P, Crepaldi L, Zhou Y, Urgo K, Dincer C, Su J, Noell G, Alasoo K, El Garwany O, Gerety SS, Newman B, Dovey OM, Parts L. Stem Cell Reports. 2023 May 9;18(5):1061-1074. doi: 10.1016/j.stemcr.2023.03.008. Epub 2023 Apr 6. PubMed |
| Multimodal perturbation analyses of cyclin-dependent kinases reveal a network of synthetic lethalities associated with cell-cycle regulation and transcriptional regulation. Ford K, Munson BP, Fong SH, Panwala R, Chu WK, Rainaldi J, Plongthongkum N, Arunachalam V, Kostrowicki J, Meluzzi D, Kreisberg JF, Jensen-Pergakes K, VanArsdale T, Paul T, Tamayo P, Zhang K, Bienkowska J, Mali P, Ideker T. Sci Rep. 2023 May 11;13(1):7678. doi: 10.1038/s41598-023-33329-2. PubMed |
| CRISPR screens decode cancer cell pathways that trigger gammadelta T cell detection. Mamedov MR, Vedova S, Freimer JW, Sahu AD, Ramesh A, Arce MM, Meringa AD, Ota M, Chen PA, Hanspers K, Nguyen VQ, Takeshima KA, Rios AC, Pritchard JK, Kuball J, Sebestyen Z, Adams EJ, Marson A. Nature. 2023 Sep;621(7977):188-195. doi: 10.1038/s41586-023-06482-x. Epub 2023 Aug 30. PubMed |
| SOD1 is a synthetic lethal target in PPM1D-mutant leukemia cells. Zhang L, Hsu JI, Braekeleer ED, Chen CW, Patel TD, Martell AG, Guzman AG, Wohlan K, Waldvogel SM, Urya H, Tovy A, Callen E, Murdaugh R, Richard R, Jansen S, Vissers L, de Vries BBA, Nussenzweig A, Huang S, Coarfa C, Anastas JN, Takahashi K, Vassiliou G, Goodell MA. bioRxiv [Preprint]. 2024 Jan 17:2023.08.31.555634. doi: 10.1101/2023.08.31.555634. PubMed |
| A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model. Kendirli A, de la Rosa C, Lammle KF, Eglseer K, Bauer IJ, Kavaka V, Winklmeier S, Zhuo L, Wichmann C, Gerdes LA, Kumpfel T, Dornmair K, Beltran E, Kerschensteiner M, Kawakami N. Nat Neurosci. 2023 Oct;26(10):1713-1725. doi: 10.1038/s41593-023-01432-2. Epub 2023 Sep 14. PubMed |
| KCNQ potassium channels modulate Wnt activity in gastro-oesophageal adenocarcinomas. Shorthouse D, Zhuang L, Rahrmann EP, Kosmidou C, Wickham Rahrmann K, Hall M, Greenwood B, Devonshire G, Gilbertson RJ, Fitzgerald RC, Hall BA. Life Sci Alliance. 2023 Sep 25;6(12):e202302124. doi: 10.26508/lsa.202302124. Print 2023 Dec. PubMed |
| A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells. Sharma S, Whitehead T, Kotowski M, Ng EZQ, Clarke J, Leitner J, Chen YL, Santos AM, Steinberger P, Davis SJ. Life Sci Alliance. 2023 Dec 7;7(3):e202302359. doi: 10.26508/lsa.202302359. Print 2024 Mar. PubMed |
| Genome-wide CRISPR screens identify the YAP/TEAD axis as a driver of persister cells in EGFR mutant lung cancer. Pfeifer M, Brammeld JS, Price S, Pilling J, Bhavsar D, Farcas A, Bateson J, Sundarrajan A, Miragaia RJ, Guan N, Arnold S, Tariq L, Grondine M, Talbot S, Guerriero ML, O'Neill DJ, Young J, Company C, Dunn S, Thorpe H, Martin MJ, Maratea K, Barrell D, Ahdesmaki M, Mettetal JT, Brownell J, McDermott U. Commun Biol. 2024 Apr 24;7(1):497. doi: 10.1038/s42003-024-06190-w. PubMed |
| Legionella pneumophila exploits the endo-lysosomal network for phagosome biogenesis by co-opting SUMOylated Rab7. Li C, Fu J, Shao S, Luo ZQ. PLoS Pathog. 2024 May 13;20(5):e1011783. doi: 10.1371/journal.ppat.1011783. eCollection 2024 May. PubMed |
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