pNvT-MHC::mCh
(Plasmid #67943)

• Purpose: Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
• Depositing Lab: Ulrich Technau
• Publication: Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):104-8. doi: 10.1073/pnas.0909148107. Epub 2009 Dec 14.

Backbone

• Vector backbone: pCRII-TOPO
• Backbone manufacturer: Invitrogen
• Vector type: transgenic reporter in the sea anemone, Nematostella vectensis
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic
• Promoter: Myosin Heavy Chain1 (MyHC1)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: mCherry-R

Resource Information

• Supplemental Documents:
  • Annotated sequence
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry