GFP-alpha-E-Catenin 3xFP4 (895)
(Plasmid #67936)

• Purpose: Mammalian expression of alpha E catenin
• Depositing Lab: Alpha Yap
• Publication: Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. doi: 10.1016/j.cub.2014.06.028. Epub 2014 Jul 24.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha E catenin
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Mutation: N-terminal domain
• Entrez Gene: Ctnna1 (a.k.a. 2010010M04Rik, Catna1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • 3xFP4 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FP4 motif is from Listeria-ActA and fused to the N terminal domain of alpha catenin and recruits Ena/VASP.