GFP-mouse vinculin full length (889)
(Plasmid #67935)

• Purpose: Mammalian expression of mouse vinculin with GFP fusion
• Depositing Lab: Alpha Yap
• Publication: Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. doi: 10.1016/j.cub.2014.06.028. Epub 2014 Jul 24.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vinculin
• Alt name: Vcl
• Alt name: movin
• Species: M. musculus (mouse)
• Insert Size (bp): 3200
• Mutation: L159P in vinculin
• Entrez Gene: Vcl (a.k.a. 9430097D22)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry