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PurposeExpresses H2B-GFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-GFP
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer FUGW-Fwd (5'-ATTACAGGGACAGCAGAGATCC-3')
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_dSV40-H2B-GFP was a gift from Ron Vale (Addgene plasmid # 67928 ; http://n2t.net/addgene:67928 ; RRID:Addgene_67928) -
For your References section:
Regulation of mRNA translation during mitosis. Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD. Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957. 10.7554/eLife.07957 PubMed 26305499