ecALDH
(Plasmid #67921)

• Purpose: Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
• Depositing Lab: Justin Siegel
• Publication: Siegel Lab ALDH Plasmids (unpublished)

Backbone

• Vector backbone: pET29b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5232
• Total vector size (bp): 6714
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Escherichia coli aldehyde dehydrogenase
• Alt name: ecALDH
• Alt name: puuC
• Species: Escherichia coli
• Insert Size (bp): 1482
• GenBank ID: AB200319.1
• Entrez Gene: puuC (a.k.a. b1300, ECK1295, JW1293, aldH)
• Promoter: T7
• Tag / Fusion Protein:
  • 6x-His (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry