rnALDH
(Plasmid
#67920)
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PurposeExpresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET29b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5232
- Total vector size (bp) 6588
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRattus norvegicus aldehyde dehydrogenase
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Alt nameAldh3a1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1356
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GenBank IDBC070924.1
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Entrez GeneAldh3a1 (a.k.a. AHDC, Ahd-c, Aldh, Aldh3)
- Promoter T7
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Tag
/ Fusion Protein
- 6x-His (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rnALDH was a gift from Justin Siegel (Addgene plasmid # 67920 ; http://n2t.net/addgene:67920 ; RRID:Addgene_67920)