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pABD933
(Plasmid #67917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Gateway® pDONR™221
  • Backbone manufacturer
    Invitrogen; life technologies Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 4761
  • Total vector size (bp) 5913
  • Vector type
    Gateway cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTG10
  • Alt name
    COLD TEMPERATURE GERMINATING 10
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1152
  • GenBank ID
    DQ666277

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 Forward (-20)
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pABD933 was a gift from Bruce Downie (Addgene plasmid # 67917 ; http://n2t.net/addgene:67917 ; RRID:Addgene_67917)

  • For your References section:

    KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1. Majee M, Kumar S, Kathare PK, Wu S, Gingerich D, Nayak NR, Salaita L, Dinkins R, Martin K, Goodin M, Dirk LMA, Lloyd TD, Zhu L, Chappell J, Hunt AG, Vierstra R, Huq E, Downie AB. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9. 10.1073/pnas.1711919115 PubMed 29632208
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