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pMAL-PIAL2M-wt
(Plasmid #67909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c2
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIAL2
  • Alt name
    AT5G41580
  • Alt name
    RING/U-box domain-containing protein
  • Species
    A. thaliana (mustard weed)
  • Mutation
    E281-A496
  • GenBank ID
    834160
  • Entrez Gene
    AT5G41580 (a.k.a. AT5G41580, MBK23.10, MBK23_10, PIAL2, PROTEIN INHIBITOR OF ACTIVATED STAT LIKE 2)
  • Promoter P-lac
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer MBP-F
  • 3′ sequencing primer M13 F20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-PIAL2M-wt was a gift from Andreas Bachmair (Addgene plasmid # 67909 ; http://n2t.net/addgene:67909 ; RRID:Addgene_67909)

  • For your References section:

    Arabidopsis PIAL1 and 2 promote SUMO chain formation as E4-type SUMO ligases and are involved in stress responses and sulfur metabolism. Tomanov K, Zeschmann A, Hermkes R, Eifler K, Ziba I, Grieco M, Novatchkova M, Hofmann K, Hesse H, Bachmair A. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300. Epub 2014 Nov 18. 10.1105/tpc.114.131300 PubMed 25415977
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