Skip to main content
Addgene

FRB-ECFP(W66A)-Giantin
(Plasmid #67903)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5468
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRB-ECFP(W66A)-Giantin
  • Species
    Synthetic
  • Insert Size (bp)
    1542
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid is modified from FRB-YFP-Giantin, published by Komatsu et al. (https://www.ncbi.nlm.nih.gov/pubmed/20154678)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-ECFP(W66A)-Giantin was a gift from Dorus Gadella (Addgene plasmid # 67903 ; http://n2t.net/addgene:67903 ; RRID:Addgene_67903)

  • For your References section:

    Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization. van_Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TWJ and Goedhart J . Scientific Reports 5, Article number: 14693 (2015) doi:10.1038/srep14693
Related items:
Commonly requested with: