Lck-FRB-ECFP(W66A)
(Plasmid #67902)

• Purpose: Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)
• Depositing Lab: Dorus Gadella
• Publication: van_Unen et al Scientific Reports 5, Article number: 14693 (2015)

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5059
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lck-FRB-ECFP(W66A)
• Species: Synthetic
• Insert Size (bp): 1125
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is obtained from Carsten Schultz (EMBL, Heidelberg). The original paper describing the plasmid is: Putyrski M. & Schultz C. (2011) Switching heterotrimeric G protein subunits with a chemical dimerizer., Chem. Biol. 18, 1126–33. https://www.ncbi.nlm.nih.gov/pubmed/21944751
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please cite the original paper for this plasmid:
Putyrski M. & Schultz C. (2011) Switching heterotrimeric G protein subunits with a chemical dimerizer., Chem. Biol. 18, 1126–33.