pYC3.60-C1
(Plasmid #67899)

• Purpose: Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells
• Depositing Lab: Dorus Gadella
• Publication: van_Unen et al Scientific Reports 5, Article number: 14693 (2015)

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YC3.60
• Alt name: Yellow Cameleon 3.60
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 1962
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is modified from RSET-YC3.60, originally reported by Nagai et al. (2004) (https://www.ncbi.nlm.nih.gov/pubmed/15247428)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry