p63(1-29)-mCherry-N1
(Plasmid #67895)

• Purpose: Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF
• Depositing Lab: Dorus Gadella
• Publication: van_Unen et al Scientific Reports 5, Article number: 14693 (2015)

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4804
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p63RhoGEF(1-29)-mCherry
• Alt name: palmitoyl-mCherry
• Species: Synthetic
• Insert Size (bp): 834
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is based on pPalmitoyl-mTurquoise2 (addgene plasmid 36209)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry