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Addgene

EGFP-Caveolin3DGV
(Plasmid #67886)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67886 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4991
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cav3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    291
  • Mutation
    Deletion of the first 53 amino acids of Caveolin3
  • GenBank ID
    NM_007617
  • Entrez Gene
    Cav3 (a.k.a. Cav-3, M-cav)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer eGFPC forward
  • 3′ sequencing primer eGFPC reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-Caveolin3DGV was a gift from Rob Parton (Addgene plasmid # 67886 ; http://n2t.net/addgene:67886 ; RRID:Addgene_67886)

  • For your References section:

    Molecular characterization of caveolin association with the Golgi complex: identification of a cis-Golgi targeting domain in the caveolin molecule. Luetterforst R, Stang E, Zorzi N, Carozzi A, Way M, Parton RG. J Cell Biol. 1999 Jun 28;145(7):1443-59. PubMed 10385524
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