pSi
(Plasmid
#67884)
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Purpose(Empty Backbone) Derivative of pSiM24 plant expression vector without the M24 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
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Backbone manufacturerStratagene, fragments of the vector were synthesized by GenArt-Life Technologies
- Backbone size (bp) 6555
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Vector typePlant Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
- 3′ sequencing primer NOSterm-R (5'-attgccaaatgtttgaacga-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The kanamycin resistance cassette is used for selection in plants.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSi was a gift from Indu Maiti (Addgene plasmid # 67884 ; http://n2t.net/addgene:67884 ; RRID:Addgene_67884) -
For your References section:
pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541
